If your AV is any good it will detect this:
- X5O!P%@AP[4\PZX54(P^)7CC)7}$ EICAR-STANDARD-ANTIVIRUS-TEST-FILE!$H+H*
Copy and paste, remove the space between the $ and the EICAR and save (so it is a 68 character file). If your AV hasn't screamed yet, ask it look at the file. If it still doesn't scream, get a new AV.
The "!" is int 21.



